Module primer_optimisation 

Deterministic bounded optimisation: NN annealing Tm and sequence-only structure screens. Numerical parameter provenance and scope: docs/primer-optimisation.md.

Structs

AssessedPrimer

DimerScreen

OptimisedGibson

OptimisedPair

PrimerConstraints

Solution

Functions

annealing_tm

Exact-match, non-self-complementary annealing duplex model. Concentrations are explicit.

optimise