Expand description
Explicit PCR-tail Gibson candidates, not thermodynamically optimised primers.
Structs§
Enums§
- Gibson
Error - Preparation
- How a fragment reaches the reaction. A
Providedfragment (restriction digest, synthesis, a stock linear DNA) is used as it is: it gets no primers, and the homology at its junctions is written into the neighbouring PCR fragments’ primers instead. - Tail
Carrier - The primer that writes a junction’s overlap into its fragment (1-based component).